Small RNA metabolism and function in Arabidopsis

Small interfering RNA; principles, applications and challenges--

Gene silencing using RNAi (RNA interference), has recently been used as a successful laboratory technique in determining the function and control of gene expression and provides a wide range of applications in molecular biology and gene therapy. RNAi is a method of suppressing gene expression. In this direction, a single-stranded RNA molecule of about 21–23 nucleotides, called siRNA (small inte...

RNA Isolation (Small RNA Extraction). Fatemeh Mehrpooyan*

Exploring Small RNA Function

The Mechanism of Small RNA Biogenesis, Degradation, and Function in <i>Arabidopsis</i>

CDC5 is a MYB-related protein that exists in plants, animals and fungi. In Arabidopsis,CDC5 regulates both growth and immunity through unknown mechanisms. Here, weshow that CDC5 from Arabidopsis positively regulates the accumulation of miRNAs thatcontrol many biological processes including development and adaptations toenvironments in plants. CDC5 interacts with both the pro...

Small RNA profiling in Chlamydomonas: insights into chloroplast RNA metabolism

In Chlamydomonas reinhardtii, regulation of chloroplast gene expression is mainly post-transcriptional. It requires nucleus-encoded trans-acting protein factors for maturation/stabilization (M factors) or translation (T factors) of specific target mRNAs. We used long- and small-RNA sequencing to generate a detailed map of the transcriptome. Clusters of sRNAs marked the 5' end of all mature mRNA...